HOXB4 But Not BMP4 Confers Self-Renewal Properties to ES-Derived Hematopoietic Progenitor Cells
نویسندگان
چکیده
منابع مشابه
HOXB4 enforces equivalent fates of ES-cell-derived and adult hematopoietic cells.
Genetic manipulation of hematopoietic stem and progenitor cells is an important tool for experimental and clinical applied hematology. However, techniques that allow for gene targeting, subsequent in vitro selection, and expansion of genetically defined clones are available only for ES cells. Such molecularly defined and, hence, "safe" clones would be highly desirable for somatic gene therapy. ...
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The homeodomain transcription factor HOXB4 is one of the most attractive tools to expand hematopoietic stem cells in vitro and in vivo and to promote the formation of hematopoietic cells from in vitro differentiated embryonic stem cells. However, the expression levels compatible with the favorable effect of enhanced self-renewal without perturbing differentiation, in vivo, remain to be determin...
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This study investigated the influence of expression of proteins of the INK4 family, particularly p16, on the growth and self-renewal kinetics of hematopoietic cells. First, retrovirus-mediated gene transfer (RMGT) was used to restore p16(INK4a) expression in the p16(INK4a)-deficient lymphoid and myeloid cell lines BV173 and K562, and it was confirmed that this inhibited their growth. Second, to...
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ژورنال
عنوان ژورنال: Transplantation
سال: 2008
ISSN: 0041-1337
DOI: 10.1097/tp.0b013e31818fe741